VZV-PCR quantitative – a kit for quantitative detection of VZV genome DNA in a test sample. Quantitative PCR kits should be used to monitor the dynamics of the disease in a patient and to evaluate the effectiveness of the selected treatment.
Varicella-zoster virus (VZV), also known as human herpesvirus 3 (HHV-3, HHV3), is one of nine known herpes viruses that can infect humans. It causes chickenpox, which usually affects children and young adults, and shingles in adults, but rarely in children. VZV infections are species-specific for humans. The virus can survive in the external environment for several hours.
Article: INQ063
Category: HERPES VIRUS INFECTIONS
During the polymerase chain reaction, sequential amplification of the VZV genome DNA (target analyte) and the human-specific genetic sequence (internal control) occurs, which leads to the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorometer of the automatic analyzer (amplifier).
The Ingeneko® VZV-PCR quantitative kit consists of the following components, designed to carry out 100 reactions:
Component | Volume | Quantity |
Buffer «VZV» | 1.0 ml | 2 pcs |
Calibrator 1 | 2.0 ml | 1 pcs |
Calibrator 2 | 2.0 ml | 1 pcs |
Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of efficiency. All components of the kit are stored at -25°C to -16°C after transportation. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided, as this may affect the efficiency of the assay.
Product advantages
High specificity
High sensitivity
Ease of use
A master mix is already prepared, allowing you to speed up the process of preparation for amplification.
Compliance with international standards
Samples have been tested in accordance with international NIBSC standards