HCV-PCR is a qualitative kit for the qualitative determination of RNA of the HCV genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Hepatitis C (also viral hepatitis C) is an infectious disease that is caused by the hepatitis C virus (HCV) and affects both the liver and other internal organs and tissues of lymphoid and non-lymphoid origin (in particular, bone marrow, thyroid gland, etc. ) - the so-called extrahepatic localization of the virus. The infection is often asymptomatic, but its chronic form leads to the formation of fibrosis in the liver and ultimately to cirrhosis or liver cancer (hepatocellular carcinoma).Infection occurs parenterally, from direct transmission of the virus from blood to blood, during intravenous drug use, or through blood transfusion due to poorly sterilized medical equipment. The virus is stored in the liver of about 85% of infected people. Hepatitis C is typically treated with antiviral therapy, and 50-80% of those treated with it recover.
Article: INS001
Category: BLOOD CONTACT INFECTIONS
During the polymerase chain reaction, the RNA of the HCV genome (target analyte) and the human-specific genetic sequence (internal control) are sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® quality HCV-PCR kit consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «HCV» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
Product benefits
