HBV-PCR quantitative – a kit for quantitative determination of HBV genome DNA in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Hepatitis B is an infection of the liver caused by the hepatitis B virus. The infection can be acute (short-term and severe) or chronic (long-term).
Hepatitis B can cause chronic infection and put people at high risk of death from cirrhosis and liver cancer.
It can be spread through contact with infected body fluids such as blood, saliva, vaginal fluids and semen. It can also be passed from mother to child.
Article: INQ002
Category: BLOOD CONTACT INFECTIONS
During the polymerase chain reaction, the DNA of the HBV genome (target analyte) and the human-specific genetic sequence (internal control) are sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The HBV-PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer "HBV" | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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