HCV-PCR quantitative

HCV-PCR quantitative – a kit for the quantitative determination of HCV genome RNA in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.

Hepatitis C (also viral hepatitis C) is an infectious disease caused by the hepatitis C virus (HCV) and affects both the liver and other internal organs and tissues of lymphoid and non-lymphoid origin (in particular, bone marrow, thyroid gland, etc.) - the so-called extrahepatic localization of the virus. The infection is often asymptomatic, but its chronic form leads to the formation of fibrosis in the liver and ultimately to cirrhosis or liver cancer (hepatocellular carcinoma). Infection occurs parenterally, from direct penetration of the virus from blood to blood, during intravenous drug use, or during blood transfusion due to poorly sterilized medical equipment. The virus is stored in the liver of about 85% of infected people. Hepatitis C is typically treated with antiviral therapy, and 50-80% of those treated with it recover.

Article: INQ001

Category: BLOOD CONTACT INFECTIONS

Principle of the method

During the polymerase chain reaction, the RNA of the HCV genome (target analyte) and the human-specific genetic sequence (internal control) are sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).

Set contents

The HCV-PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:

Storage conditions

All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.