Ureaplasma urealyticum-PCR quantitative - a kit for quantitative determination of the DNA of the Ureaplasma urealyticum genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Ureaplasma urealyticum is a species of Ureaplasma, a genus of bacteria belonging to the family Mycoplasmataceae. Ureaplasma parvum is a uterine commensal, a member of the uterine microbiome, in healthy women of reproductive age. In addition, this pathogen can latently infect the chorionic villi tissue of pregnant women, thus affecting the outcome of pregnancy.
Article: INQ036
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, the DNA of the Ureaplasma urealyticum genome (target analyte) and the human-specific genetic sequence (internal control) is sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ureaplasma urealyticum-PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Ureaplasma urealyticum» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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