Ureaplasma parvum-PCR quantitative - a kit for quantitative determination of the DNA of the Ureaplasma parvum genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Ureaplasma parvum is a species of Ureaplasma, a genus of bacteria belonging to the family Mycoplasmataceae. Ureaplasma parvum is a uterine commensal, a member of the uterine microbiome, in healthy women of reproductive age. In addition, this pathogen can latently infect the chorionic villi tissue of pregnant women, thus affecting the outcome of pregnancy.
Article: INQ035
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, the DNA of the Ureaplasma parvum genome (target analyte) and the human-specific genetic sequence (internal control) is sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ureaplasma parvum–PCR quantitative Ingeneko® kit consists of the following components, designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «Ureaplasma parvum» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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