Toxoplasma-PCR quantitative – a kit for quantitative determination of DNA of the Toxoplasma gondii genome in the test sample. Quantitative PCR kits should be used to track the dynamics of the disease in the patient and assess the effectiveness of the selected treatment.
Toxoplasma is a type of apicomplexan parasite, the main carrier of which are cats, but can also be carried by some other warm-blooded animals. Toxoplasmosis is a disease caused by toxoplasma, usually mild. However, for the fetus, if the mother became infected with toxoplasmosis during pregnancy, as well as for a person with reduced immunity, this disease can have serious consequences, including death.
Article: INQ080
Category: TORCH INFECTIONS
During the polymerase chain reaction, sequential amplification of the DNA of the Toxoplasma gondii genome (target analyte) and the human-specific genetic sequence (internal control) occurs, which leads to the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorometer of the automatic analyzer (amplifier).
The "Toxoplasma-PCR quantitative Ingeneko®" kit consists of the following components, designed to carry out 100 reactions:
Component | Volume | Quantity |
Buffer «Toxoplasma» | 1.0 ml | 2 pcs |
Calibrator 1 | 2.0 ml | 1 pcs |
Calibrator 2 | 2.0 ml | 1 pcs |
Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of efficiency. All components of the kit are stored at -25°C to -16°C after transportation. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided, as this may affect the efficiency of the assay.
Product advantages
High specificity
High sensitivity
Ease of use
A master mix is already prepared, allowing you to speed up the process of preparation for amplification.
Compliance with international standards
Samples have been tested in accordance with international NIBSC standards
