SARS-CoV-2-PCR quantitative - a kit for the quantitative determination of RNA of the SARS-CoV-2 genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
SARS-CoV-2, a single-stranded RNA-containing strain of the SARSr-CoV species of the beta-coronavirus genus, was first genetically discovered in 2019 in a sample of a patient with SARS during the pneumonia outbreak in Wuhan 2019 - 2020. According to the World Health Organization (WHO) ), transmission of this virus is possible from person to person through airborne droplets within the families of patients, so more outbreaks are possible.
Article: INQ050
Category: RESPIRATORY INFECTIONS
During the polymerase chain reaction, the RNA of the SARS-CoV-2 genome (target analyte) and the human-specific genetic sequence (internal control) are sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The SARS-CoV-2-PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «SARS-CoV-2» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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