SARS-CoV-2-PCR is a qualitative kit for the qualitative determination of the RNA of the SARS-CoV-2 genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
SARS-CoV-2, a single-stranded RNA-containing strain of the SARSr-CoV species of the betaconavirus genus, was first genetically discovered in 2019 in a sample of a patient with atypical pneumonia during the pneumonia outbreak in Wuhan in 2019–2020. According to the World Health Organization (WHO), transmission of this virus is possible from person to person through respiratory droplets within the families of patients, so more outbreaks are possible.
Article: INS050
Category: RESPIRATORY INFECTIONS
During the polymerase chain reaction, the RNA of the SARS-CoV-2 genome (target analyte) and the human-specific genetic sequence (internal control) are sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The qualitative Ingeneko® SARS-CoV-2-PCR kit consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «SARS-CoV-2» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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