Neisseria gonorrhoeae-PCR quantitative – kit for quantitative determination of Neisseria gonorrhoeae genome DNA in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Gonococcus (Neisseria gonorrhoeae) is the causative agent of acute inflammation of the mucous membranes of the genitourinary tract - gonorrhea and inflammation of the eyes in newborns, which leads to blindness - blenorrhea.
Article: INQ031
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, the DNA of the Neisseria gonorrhoeae genome (target analyte) and the human-specific genetic sequence (internal control) is sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Neisseria gonorrhoeae–PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Neisseria gonorrhoeae» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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