Neisseria gonorrhoeae-PCR is a qualitative kit for the qualitative determination of the DNA of the Neisseria gonorrhoeae genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Gonococcus (Neisseria gonorrhoeae) is the causative agent of acute inflammation of the mucous membranes of the genitourinary tract - gonorrhea and inflammation of the eyes in newborns, which leads to blindness - blenorrhea.
Article: INS031
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, the DNA of the Neisseria gonorrhoeae genome (target analyte) and the human-specific genetic sequence (internal control) is sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
Neisseria gonorrhoeae-PCR quality kit Ingeneko® consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «Neisseria gonorrhoeae» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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