Mycoplasma hominis-PCR quantitative - a kit for quantitative determination of the DNA of the Mycoplasma hominis genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Mycoplasma hominis is a species of microbes of the genus Mycoplasma. M. hominis has the ability to penetrate human cells. Mycoplasmas, along with ureaplasmas, are the smallest known free-living organisms. Mycoplasma hominis causes pelvic inflammatory disease and bacterial vaginosis. Also causes male infertility.
Article: INQ034
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, sequential amplification of Mycoplasma hominis genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® Mycoplasma hominis-PCR quantitative kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Mycoplasma hominis» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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