Mycoplasma hominis-PCR is a qualitative kit for the qualitative determination of the DNA of the Mycoplasma hominis genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Mycoplasma hominis is a species of microbes of the genus Mycoplasma. M. hominis has the ability to penetrate human cells. Mycoplasmas, along with ureaplasmas, are the smallest known free-living organisms. Mycoplasma hominis causes pelvic inflammatory disease and bacterial vaginosis. Also causes male infertility.
Article: INS034
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, sequential amplification of Mycoplasma hominis genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® quality Mycoplasma hominis-PCR kit consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «Mycoplasma hominis» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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