Mycoplasma genitalium-PCR quantitative - a kit for quantitative determination of the DNA of the Mycoplasma genitalium genome in the test sample. Quantitative PCR kits should be used to track the progress of a patient's disease and evaluate the effectiveness of selected treatments.
Mycoplasma genitalium is a small pathogenic sexually transmitted bacterium that lives on the mucous epithelial cells of the human urinary and genital tract. Resistant to many antibiotics, including the macrolide azithromycin, which has recently been the most reliable treatment.
Article: INQ033
Category: SEXUALLY TRANSMITTED INFECTIONS
During the polymerase chain reaction, sequential amplification of Mycoplasma genitalium genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® Mycoplasma genitalium-PCR quantitative kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Mycoplasma genitalium» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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