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Mycobacterium tuberculosis-PCR quantitative
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Mycobacterium tuberculosis-PCR quantitative

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Brand :
Індженіко
Country:
Ukraine

Mycobacterium tuberculosis-PCR quantitative – a kit for quantitative determination of the DNA of the Mycobacterium tuberculosis genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.

Mycobacterium tuberculosis is a bacterium that causes a significant number of cases of tuberculosis in humans.

Article: INQ015

Category: RESPIRATORY INFECTIONS

Principle of the method

During the polymerase chain reaction, sequential amplification of Mycobacterium tuberculosis genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).

Set contents

The Ingeneko® Mycobacterium tuberculosis-PCR quantitative kit consists of the following components for 100 reactions:

Component Volume Quantity
Buffer «Mycobacterium tuberculosis» 1.0 ml 2 pcs
Calibrator 1 2.0 ml 1 pcs
Calibrator 2 2.0 ml 1 pcs
Negative standard 2.0 ml 1 pcs

Storage conditions

All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.

Product benefits

  • High specificity, no cross-reaction
  • High sensitivity
  • Ease of use
  • Compliance with international standards
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