Mycobacterium tuberculosis-PCR quantitative – a kit for quantitative determination of the DNA of the Mycobacterium tuberculosis genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Mycobacterium tuberculosis is a bacterium that causes a significant number of cases of tuberculosis in humans.
Article: INQ015
Category: RESPIRATORY INFECTIONS
During the polymerase chain reaction, sequential amplification of Mycobacterium tuberculosis genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® Mycobacterium tuberculosis-PCR quantitative kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Mycobacterium tuberculosis» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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