Mycobacterium tuberculosis-PCR is a qualitative kit for the qualitative determination of the DNA of the Mycobacterium tuberculosis genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Mycobacterium tuberculosis is a bacterium that is responsible for a significant number of cases of tuberculosis in humans.
Article: INS015
Category: RESPIRATORY INFECTIONS
During the polymerase chain reaction, sequential amplification of Mycobacterium tuberculosis genomic DNA (target analyte) and human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Ingeneko® quality Mycobacterium tuberculosis-PCR kit consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «Mycobacterium tuberculosis» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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