HHV 6/7-PCR quantitative – a kit for the quantitative determination of HHV 6/7 genome DNA in the test sample. Quantitative PCR kits should be used to track the dynamics of the disease in a patient and to evaluate the effectiveness of the selected treatment.
Human herpesvirus 6 infection is an infectious disease from the group of herpesvirus infections, which is caused by human herpesvirus type 6 (HHV-6). In the acute form, it most often occurs in children as a sudden exanthema (roseola infantum, exanthema subitum, 6th childhood rash disease), less often with the development of lesions of the connective tissue and the blood system. In the chronic course, lesions of the liver, lymph nodes, lungs, nervous system, the development of malignant neoplasms of the lymphatic system and immunodeficiency are possible.
Infection caused by human herpesvirus type 7 - lesions caused by human herpesvirus type 7 (HHV-7). It is part of the herpesvirus infection group. Like most herpesvirus infections, it is an anthroponotic disease.
HHV-7, together with HHV-6, is considered the cause of sudden exanthema in children, pink lichen. In general, there is an assumption that HHV-7 almost always infects people together with HHV-6, after which they act together.
Article: INQ067
Category: HERPES VIRUS INFECTIONS
During the polymerase chain reaction, sequential amplification of the DNA of the HHV 6, HHV 7 genome (target analytes) and the human-specific genetic sequence (internal control) occurs, which leads to the release of specific fluorophores (FAM), (ROX) and (HEX), respectively. Their fluorescence is recorded using a photofluorometer of the automatic analyzer (amplifier).
The "HHV 6/7-PCR quantitative Ingeneko®" kit consists of the following components, designed to carry out 100 reactions:
Component | Volume | Quantity |
Buffer «HHV 6/7» | 1.0 ml | 2 pcs |
Calibrator 1 | 2.0 ml | 1 pcs |
Calibrator 2 | 2.0 ml | 1 pcs |
Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of efficiency. All components of the kit are stored at -25°C to -16°C after transportation. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided, as this may affect the efficiency of the assay.
Product advantages
High specificity
High sensitivity
Ease of use
A master mix is already prepared, allowing you to speed up the process of preparation for amplification.
Compliance with international standards
Samples have been tested in accordance with international NIBSC standards
