HSV 1/2 - PCR QUALITATIVE

HSV 1/2-PCR quantitative – a kit for the quantitative determination of HSV 1/2 genome DNA in the test sample. Quantitative PCR kits should be used to track the dynamics of the disease in a patient and assess the effectiveness of the selected treatment.

Herpes simplex virus types 1 and 2 are a type of virus from the Herpesviridae family. They are the causative agents of herpes simplex in people with various clinical forms of the course. They are extremely common human pathogens that cause lifelong infections. Herpes simplex virus type 1 (HSV-1) causes mainly lesions of the skin, mucous membranes of the oropharynx, while herpes simplex virus type 2 (HSV-2) mainly affects the genitourinary organs. The infectivity of both types is extremely high.

Article: INQ061

Category: HERPES VIRUS INFECTIONS

Method principle

During the polymerase chain reaction, sequential amplification of the HSV 1/2 genome DNA (target analyte) and the human-specific genetic sequence (internal control) occurs, which leads to the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorometer of the automatic analyzer (amplifier).

Storage conditions

All components of the kit can be stored for 1 year from the date of manufacture without loss of efficiency. All components of the kit are stored at -25°C to -16°C after transportation. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided, as this may affect the efficiency of the assay.

Kit composition

The Ingeneko® HSV 1/2-PCR quantitative kit consists of the following components, designed to carry out 100 reactions: