HPV 16/18/45-PCR is a qualitative kit for the qualitative determination of the DNA of the HPV 16/18/45 genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Human papillomavirus (HPV), Human papillomavirus (HPV) belongs to the papovaviridae family, a genus of papillomaviruses. This is a DNA virus, heat stable. It is the most common sexually transmitted infection: according to some data, up to 80% of the population is infected at a young age. The virus shows affinity for the epithelium of the skin, genital mucosa and other organs (larynx, oral cavity, conjunctival mucosa).
HPV of high carcinogenic risk (HPV HCR): the group of types of high carcinogenic risk is represented by 15 genotypes, the most common are ten: 16, 18, 31, 33, 35, 39, 45, 52, 58, 59. At the same time, the share of 16 and 18 types account for 70% of all cases of infection, and it is these types that are characterized by a high carcinogenic potential; in relation to genotype 18, a high frequency of detection is observed in adenocarcinomas. To date, the role of HPV has been proven in the development of cervical cancer, a large proportion of anal cancer (about 90% of cases), about 40% of all cases of cancer of the vagina, vulva, penis, 10–15% of cases of cancer of the oral cavity and larynx.
Article: INM040
Category: HERPES VIRUS INFECTIONS
During the polymerase chain reaction, the DNA of the HPV 16/18/45 genome (target analyte) and the human-specific genetic sequence (internal control) is sequentially amplified, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The high-quality Ingeneko® HPV 16/18/45-PCR kit consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «HPV 16/18/45» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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