HAV-PCR quantitative – a kit for the quantitative determination of HAV genome DNA in the test sample. Quantitative PCR kits should be used to track the dynamics of the disease in a patient and to assess the effectiveness of the selected treatment.
Hepatitis A is an acute infectious disease with liver damage, transmitted by the feco-oral mechanism. The disease is common in all countries of the world [3], but due to the availability of a highly effective vaccine in many countries with developed medicine, the incidence has been reduced to almost zero. In third world countries, children and young people are mainly affected.
Article: INQ007
Category: BLOOD-CONTACT INFECTIONS
During the polymerase chain reaction, sequential amplification of the HAV genome DNA (target analyte) and the human-specific genetic sequence (internal control) occurs, which leads to the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorometer of the automatic analyzer (amplifier).
All components of the kit can be stored for 1 year from the date of manufacture without loss of efficiency. All components of the kit are stored at -25°C to -16°C after transportation. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided, as this may affect the efficiency of the assay.
The Ingeneko® HAV-PCR quantitative kit consists of the following components, designed to carry out 100 reactions:
Component | Volume | Quantity |
Buffer «HAV» | 1.0 ml | 2 pcs |
Calibrator 1 | 2.0 ml | 1 pcs |
Calibrator 2 | 2.0 ml | 1 pcs |
Negative standard | 2.0 ml | 1 pcs |
Product advantages
High specificity
High sensitivity
Ease of use
A master mix is already prepared, allowing you to speed up the process of preparation for amplification.
Compliance with international standards
Samples have been tested in accordance with international NIBSC standards
