Candida albicans-PCR quantitative – a kit for quantitative determination of the DNA of the Candida albicans genome in the test sample. Quantitative PCR kits should be used to track a patient's disease progression and evaluate the effectiveness of selected treatments.
Candida albicans is a diploid fungus (a form of yeast-like fungi), capable of mating, but not in the form of meiosis, the causative agent of opportunistic infections in humans, transmitted by the fecal-oral or sexual route. Systemic fungal infections (fungemias) are important causes of morbidity and mortality in patients with immunodeficiency (eg, AIDS, cancer chemotherapy, or organ transplantation). In addition, this fungus is an important causative agent of hospital-borne infections.
Article: INQ039
Category: UROGENITAL INFECTIONS
During the polymerase chain reaction, sequential amplification of the Candida albicans genome (target analyte) and the human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Candida albicans-PCR quantitative Ingeneko® kit consists of the following components for 100 reactions:
| Component | Volume | Quantity |
| Buffer «Candida albicans» | 1.0 ml | 2 pcs |
| Calibrator 1 | 2.0 ml | 1 pcs |
| Calibrator 2 | 2.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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