Candida albicans-PCR is a qualitative kit for the qualitative determination of the DNA of the Candida albicans genome in the test sample. Qualitative PCR kits are used to determine whether an infectious agent is present in a sample.
Candida albicans is a diploid fungus (a form of yeast-like fungi), capable of mating, but not in the form of meiosis, the causative agent of opportunistic infections in humans, transmitted by the fecal-oral or sexual route. Systemic fungal infections (fungemias) are important causes of morbidity and mortality in patients with immunodeficiency (eg, AIDS, cancer chemotherapy, or organ transplantation). In addition, this fungus is an important causative agent of hospital-borne infections.
Article: INS039
Category: UROGENITAL INFECTIONS
During the polymerase chain reaction, sequential amplification of the Candida albicans genome (target analyte) and the human-specific genetic sequence (internal control) occurs, resulting in the release of specific fluorophores (FAM) and (HEX), respectively. Their fluorescence is recorded using a photofluorimeter of an automatic analyzer (amplifier).
The Candida albicans-PCR quality kit Ingeneko® consists of the following components designed to carry out 100 reactions:
| Component | Volume | Quantity |
| Buffer «Candida albicans» | 1.0 ml | 2 pcs |
| Positive standard | 1.0 ml | 1 pcs |
| Negative standard | 2.0 ml | 1 pcs |
All components of the kit can be stored for 1 year from the date of manufacture without loss of effectiveness. All kit components are stored at -25°C to -16°C after transport. Repeated thawing and freezing (> 2 times) of the master mix and positive control should be avoided as this may affect assay performance.
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